METHOD OF FREEZING AQUEOUS SOLUTIONS OF GEL-FORMING BIOLOGICAL SUBSTANCES UNTIL AMORPHOUS STATE IS ACHIEVED AND MELTING AMORPHOUS SPECIMENS OF THESE SUBSTANCES Russian patent published in 1998 - IPC

FIELD: biological procedures. SUBSTANCE: method can be used in preparing amorphous state of biological materials, including live cells. It allows melting amorphous specimens without crystallization. Specimen in dielectric container is placed in high-pressure chamber with a liquid dielectric medium transmitting hydrostatic pressure on the specimen at a temperature below crystallization temperature. Chamber pressure is then raised to 100 atm and rotating pulse electric field is imposed on specimen. Amorphous specimen is heated to crystallization temperature to melt amorphous structure, whereas rotating pulse electric field hampers formation of crystalline ice structure. Further heating of amorphous to positive temperatures leads to melting of specimen without crystallization. EFFECT: avoided crystallization of specimens. 3 dwg