FIELD: biotechnology. SUBSTANCE: human recombinant IL-2 exhibiting natural amino acid sequence but containing reduced cysteine residues at positions 58, 105 and 125 is obtained. IL-2 is obtained from "inclusion bodies" formed as result of recombinant vector expression containing human native IL-2 gene in bacterial cells. Method involves solubilization in reducing medium with chaotropic agent followed by purification by precipitation, reverse phase high pressure liquid chromatography (HPLC), dilution of IL-2-containing fraction with acid medium and repeated reverse phase HPLC. Specific activity of obtained IL-2 is at least 0.5 x 107 U/mg. EFFECT: improved method of preparing, high specific activity. 2 cl, 9 dwg, 3 tbl
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