VECTOR PMT440 AT POSITIVE SELECTION FOR FOREIGN DNA FRAGMENTS CLONING Russian patent published in 1998 - IPC

Abstract RU 2105064 C1

FIELD: molecular biology, genetic engineering. SUBSTANCE: vector has barnase structural gene subcloned from Bacillus amyloliquefaciens under control of synthetic tac-promoter; universal polylinker of plasmid pHC19 consisting of 45 b. p. inserted in barnase gene instead of valine-36; phoA-signal sequence from Escherichia coli; gene of specific barnase inhibitor - barstar under its own promoter; plasmid pHC19 fragment including replication start site ori, gene A_mp r.. Restriction sites are located at the following positions from replication start point of plasmid DNA, b. p.: Eco RI 300; SacI 306; KpuI 312; SmaI 316; Bam HI 321; SalI 333; PstI 339; SphI 345, and HindIII 351. Vector size is 2936 b. p., its capacity is 6 t.b.p. Vector provides positive selection of cloning fragments of foreign DNA in different strains of E. coli. EFFECT: improved method of selection. 1 dwg

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RU 2 105 064 C1

Authors

Deev S.M.

Jazynin S.A.

Dates

1998-02-20—Published

1996-07-19—Filed