FIELD: biotechnology, genetic engineering. SUBSTANCE: invention relates to a new polypeptide eliciting urate oxidase activity which is obtained by culturing strains transformed with recombinant plasmid DNAs containing DNA fragment encoding urate oxidase with established amino acid sequence being that methionine precedes not obligatory. Specific activity of urate oxidase is 16 U/mg, not less, molecular mass is 33.5 kDa and isoelectric point is about 8.0. Method of polypeptide producing involves the use of strain of bacterium Escherichia coli K12 RR I/p466, or yeast strains Saccharomyces cerevisiae CNCM I-920 or CNCM I-919, or strain of cultured mammalian cells COS/pSV 860. EFFECT: improved method of polypeptide producing. 16 cl, 14 tbl, 16 dwg
| Title |
Year |
Author |
Number |
| DNA FRAGMENT, RECOMBINANT POLYPEPTIDE EXHIBITING ANTITHROMBIN ACTIVITY, METHOD OF ITS PREPARING (VARIANTS), PHARMACEUTICAL COMPOSITION, RECOMBINANT VECTOR (VARIANTS) |
1992 |
- Paolo Sarmentos
- Filipp De Taksis Dju Poet
- Dzhampaolo Nitti
- Ehmanuehla Skacheri
|
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1990 |
- Robert Frantsiskus Marija Van Gorkom
- Villem Van Khartingsveldt
- Petrus Andreas Van Paridon
- Annemari Ehvelin Vehnstra
- Rudol'F Gejsbertus Mari Lejten
- Gerardus Kornelis Marija Selten
|
RU2113468C1 |
| METHOD OF PRODUCING S-(+)-2,2-DIMETHYLCYCLOPROPANE CARBOXAMIDE, DNA FRAGMENT ENCODING R-(-)-2,2-DIMETHYLCYCLOPROPANE- -CARBOXAMIDE HYDROLASE OF COMAMONAS, RECOMBINANT PLASMID DNA (VARIANTS), STRAIN OF BACTERIUM ESCHERICHIA COLI (VARIANTS) |
1992 |
- Tomas Tsimmermann
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- Ehlizabet Belen
|
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1989 |
|
RU2228357C2 |
| METHOD OF PREPARING THE HETEROLOGIC POLYPEPTIDE IN EUCARYOTIC MICROORGANISMS |
1986 |
|
RU2091490C1 |
| DNA FRAGMENT ENCODING IMMUNOGENIC SURFACE MEROSOITE POLYPEPTIDE, IMMUNOGENIC POLYPEPTIDE (VARIANTS) AND A METHOD OF ITS PREPARING, RECOMBINANT PLASMID DNA AND A METHOD OF ITS PREPARING, A METHOD OF PREPARING RECOMBINANT VIRUS OF VACCINE, A METHOD OF PREPARING MICROORGANISM, VACCINE AGAINST COCCIDIOSIS IN POULTRY |
1992 |
- Mehri-Khelen Binger
- Luis Pazamontes
|
RU2130072C1 |
| DNA S54 FRAGMENT ENCODING POLYPEPTIDE SHOWING ACTIVITY OF GAMMA-INTERFERON, RECOMBINANT PLASMID DNA PROVIDING THE SYNTHESIS OF POLYPEPTIDE WITH ACTIVITY OF GAMMA-INTERFERON, AND THE STRAIN OF ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE WITH GAMMA-INTERFERON ACTIVITY |
1992 |
- Tat'Kov S.I.
- Smirnova O.Ju.
- Kishchenko G.P.
- Petrenko V.A.
|
RU2046144C1 |
| RECOMBINANT POLYPEPTIDE, METHOD OF INHIBITION OF PLATELET AGGREGATION, RECOMBINANT PLASMID DNA (VARIANTS), RECOMBINANT STRAIN OF ESCHERICHIA COLI (VARIANTS), METHOD OF POLYPEPTIDE PREPARING |
1991 |
- Leonard Garfinkel'[Us]
- Tamar Rikhter[Il]
|
RU2109816C1 |
| RECOMBINATION PLT 9 PLASMIDE DNA WHICH CODES POLYPEPTIDE POSSESSING PROPERTIES OF HUMAN LYMPHOTOXIN, RECOMBINATION PLASMIDE PLT 16 DNA USED FOR CONSTRUCTING PLT 9 DNA, AND 'ESCHERICHIA COLI' BACTERIUM STRAIN - PRODUCER OF POLYPEPTIDE HAVING QUALITIES OF HUMAN LYMPHOTOXIN |
1988 |
- Korobko V.G.
- Dobrynin V.N.
- Filippov S.A.
- Chumakov A.M.
- Panina A.A.
- Boldyreva E.F.
- Nedospasov S.A.
- Shakhov A.N.
- Turetskaja R.L.
|
SU1561510A1 |
| METHOD OF PRODUCING BACILLUS STRAIN CONTAINING TWO COPIES OF DNA SEQUENCE ENCODING ENZYME EXHIBITING HYDROLASE ACTIVITY |
1988 |
- Kristian A.G. Van Ehkelen[Nl]
- Jokhannes K.Vvan Der Lan[Nl]
- Leonardus Dzh.S.M. Mjulleners[Nl]
|
RU2091487C1 |
