FIELD: medicine. SUBSTANCE: method of selective isolation of pure cultures of propane-oxidizing rhodococci is based upon their resistance to a number of antibiotic substances under conditions of propanemonooxygenous activity induction. To obtain better isolation of propane-oxidizing rhodococci into mineral medium that contains potassium nitrate, monopotassium phosphate, disubstituted potassium phosphate, sodium chloride, magnesium sulfate, calcium chloride, ferric chloride one should add propane as the single source of carbon and antibiotics and their combinations at optimal concentrations as a selective agent that suppresses the growth of accompanying eubacteria. EFFECT: increased selective index (85-98%) which enables to reveal maximal amount of cultures of propane-oxidizing rhodococci in a sample tested, higher efficiency of the method to be used in ecological trials and at cultivation of combined cultures under nonsterile conditions. 4 cl, 3 tbl, 1 dwg
