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2 108 343

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IPC

(21) (22)

Application

95112676/13, 1995-07-20

(22)

Actual filing date

1995-07-20

(45)

Published

1998-04-10

(72)

Inventor

Nikitina Z.K.Spivak S.M.Veshchikova E.V.Bykov V.A.

(73)

Holder

Nauchno-Issledovatel'Skij Tsentr Biologicheskikh Struktur Nauchno-Proizvodstvennoe Ob"Edinenie

METHOD OF ISOLATION AND PURIFICATION OF HUMAN EPIDERMAL GROWTH FACTOR (H-EGF) Russian patent published in 1998 - IPC

Abstract RU 2108343 C1

FIELD: medicinal biotechnology. SUBSTANCE: growth factor is isolated from cultural fluid of recombinant strains. Chromatography separation is carried out on CNBr-activated Sepharose bound with polyclonal antibodies to H-EGF. The end product is eluted with 1 M acetic acid. Method ensures to prepare growth factor by a single-step process at purity degree 99%, not less. EFFECT: improved method of preparing. 1 tbl, 4 dwg

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RU 2 108 343 C1

Authors

Nikitina Z.K.

Spivak S.M.

Veshchikova E.V.

Bykov V.A.

Dates

1998-04-10—Published

1995-07-20—Filed