Show metadata Hide metadata

(19)

(11)

2 553 533

(13)

(51)

IPC

C07K1/16(2006-01-01)

C12N9/72(2006-01-01)

(21) (22)

Application

2013147050/10, 2013-10-23

(24)

Start date

2013-10-23

(22)

Actual filing date

2013-10-23

(45)

Published

2015-06-20

(72)

Inventor

Tikhonov Roman VladimirovichSmolova Ksenija AleksandrovnaKazarov Aleksandr AleksandrovichFeshin Denis BorisovichFabrichnyj Igor' Pavlovich

(73)

Holder

Obshchestvo S Ogranichennoj Otvetstvennost'Ju Biotekhnologicheskij Tsentr

METHOD OF EXTRACTION AND PURIFICATION OF RECOMBINANT HUMAN PROUROKINASE M5 Russian patent published in 2015 - IPC C07K1/16 C12N9/72

Abstract RU 2553533 C2

FIELD: biotechnology.

SUBSTANCE: method comprises the steps of destroying the bodies of inclusion, renaturation and purification of protein. Before renaturation, the preliminary purification of protein is carried out using chromatography on Q-Sepharose and SP-Sepharose using the combined columns with Q- and SP-Sepharose. After renaturation chelate and ion-exchange chromatography of recombinant prourokinase M5 is carried out without intermediate elution of the target protein with use of metal-chelate sorbent activated with ions Co²⁺or Zn²⁺.

EFFECT: invention enables to select prourokinase M5 from inclusion bodies, comprising the present prourokinase, with high yield.

4 cl, 2 tbl, 4 ex

Similar patents RU2553533C2

Title	Year	Author	Number
METHOD OF PURIFICATION OF RECOMBINANT GRANULOCYTE COLONY-STIMULATING HUMAN FACTOR	2011	Grigor'Ev Pavel Igorevich Selishchev Stepan Vladimirovich	RU2440417C1
METHOD FOR OBTAINING ACTIVE STARTING OF VACCINE AGAINST ROTAVIRUS INFECTION OF PROTEIN FLiCVP6VP8	2017	Bogomolova Elena Grigorevna Dukhovlinov Ilya Vladimirovich Dobrovolskaya Olga Andreevna Fedorova Ekaterina Alekseevna Ishchuk Sergej Aleksandrovich Simbirtsev Andrej Semenovich	RU2649132C1
METHOD FOR PREPARING GENETIC-ENGINEERING HUMAN INSULIN	2006	Bairamashvili Dmitrij Il'Ich Gusarov Dmitrij Alekseevich Davydov Vladimir Leonidovich Zinchenko Aleksej Alekseevich Kosarev Sergej Anatol'Evich Lasman Vladimir Aleksandrovich Miroshnikov Anatolij Ivanovich Mikhalev Aleksej Vladimirovich Shibanova Elena Dmitrievna	RU2322504C1
METHOD FOR COMMERCIAL PRODUCTION AND PURIFICATION OF HUMAN RECOMBINANT GROWTH HORMONE OF INCLUSION BODIES	2011	Bobruskin Aleksej Igorevich Kononova Natal'Ja Vjacheslavovna Mart'Janov Vitalij Afanas'Evich Shuster Aleksandr Mikhajlovich	RU2473556C1
STRAIN OF BACTERIA Escherichia coli JM109/pHINS21 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN AND METHOD FOR PRODUCTION OF HUMAN PROINSULIN	2007	Rodionov Petr Ivanovich Rodionov Petr Petrovich Shmatchenko Vadim Vasil'Evich Stepanov Aleksej Vjacheslavovich Bajdus' Aleksandr Nikolaevich Shmatchenko Natal'Ja Anatol'Evna Gorkun Taras Alekseevich	RU2376368C2
METHOD OF RECOMBINANT HUMAN INSULIN PREPARING	1999	Kalinin Ju.T. Urakov N.N. Ivanov V.T. Stepanov A.V. Miroshnikov A.I. Gavrikov V.G. Bairamashvili D.I. Donetskij I.A. Korobko V.G. Kostromina T.I. Guljaev V.A. Litvinenko M.A. Bajdus' A.N. Korobov V.I. Krasil'Nikov V.A. Borisov N.V. Shmatchenko N.A. Vorotnikova I.I. Gorkun O.G. Derbyshev V.I. Nozdrin V.N. Dunajtsev I.A. Lipin M.Ju. Bekmuratova I.I. Kobeleva V.A. Shepelin A.P. Martovetskij M.N. Fedjukin V.S. Abrosimova L.I.	RU2141531C1
METHOD OF PRODUCING RECOMBINANT HUMAN PROINSULIN C-PEPTIDE	2007	Rodionov Petr Ivanovich Rodionov Petr Petrovich Shmatchenko Vadim Vasil'Evich Stepanov Aleksej Vjacheslavovich Bajdus' Aleksandr Nikolaevich Borisov Nikolaj Viktorovich	RU2451750C2
METHOD FOR LARGE-SCALE PRODUCTION, SEPARATION AND PURIFICATION OF RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR	2010	Chugunov Aleksandr Mikhajlovich Skatova Galina Evgen'Evna Morozov Dmitrij Valentinovich Efanov Jurij Georgievich Denisov Lev Aleksandrovich Goncharova Ol'Ga Vladimirovna Chernovskaja Tat'Jana Veniaminovna Morozova Elena Leonidovna	RU2487885C2
RECOMBINANT PLASMID DNA pHINS11 CODING HYBRID PROTEIN-HUMAN INSULIN PRECURSOR, Escherichia coli CELL, TRANSFORMED WITH RECOMBINANT PLASMID DNA pHINS11, Escherichia coli BACTERIA STRAIN JM109/pHINS11 - PRODUCER OF HYBRID PROTEIN-HUMAN INSULIN PRECURSOR, AND METHOD OF OBTAINING HUMAN INSULIN	2006	Shmatchenko Vadim Vasil'Evich Shmatchenko Natal'Ja Anatol'Evna Bajdus' Aleksandr Nikolaevich Kuptsov Vasilij Nikolaevich Nozdrin Viktor Nikolaevich Stepanov Aleksej Vjacheslavovich	RU2354702C2
RECOMBINANT PLASMID DNA p280_2GM CODING POLYPEPTIDE WITH PROPERTIES OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR OF HUMAN, STRAIN ESCHERICHIA COLI SG 20050/p280_2GM - PRODUCER OF POLYPEPTIDE WITH PROPERTIES OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR OF HUMAN AND METHOD OF OBTAINING OF SAID POLYPEPTIDE	2018	Gileva Irina Pavlovna Esina Tatyana Igorevna Volosnikova Ekaterina Aleksandrovna Gogina Yana Stanislavovna Lebedev Leonid Rudolfovich Danilenko Elena Dmitrievna	RU2708556C1

RU 2 553 533 C2

Authors

Tikhonov Roman Vladimirovich

Smolova Ksenija Aleksandrovna

Kazarov Aleksandr Aleksandrovich

Feshin Denis Borisovich

Fabrichnyj Igor' Pavlovich

Dates

2015-06-20—Published

2013-10-23—Filed