FIELD: microbiological industry. SUBSTANCE: invention relates to production of bacterial agents for plant protection, in part, bioinsecticide to coleopterus insects. Strain of bacterium Bacillus thuringiensis isolated from the natural environment is a producer of endotoxin for control of Colorado potato beetle. Strain has a number 16931 and deposited in VKPM at number B-6649. Cultural fluid of this strain (dilution is 1:10) causes 100% lethal effect on Colorado potato beetle larvae of I-II instars. For producing endotoxin nutrient medium is prepared, sown its with strain, cultured in fermenter up to sporulation onset. Then obtained cultural fluid is taken off (90-95% of volume), additional fermentation is carried out at the room temperature followed by centrifugation and preparing supernatant and paste. Paste is used for the end product making. Supernatant is fed to preparing new nutrient medium and residue of cultural fluid in fermenter is used as sowing material. EFFECT: strain indicated above, improved method of endotoxin preparing. 2 cl, 1 tbl
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