FIELD: biotechnology, microbiology. SUBSTANCE: method involves growing the culture-producer, extraction of the end product with organic solvent mixing with water, repeated extraction of the obtained primary extract with organic solvent no mixing with water under alkaline conditions. Chromatographic purification is carried out at isocratic regime using system chloroform : methanol at ratio (80-100):1. EFFECT: improved method of preparing. 13 cl
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Authors
Dates
1998-04-20—Published
1994-07-05—Filed