FIELD: biotechnology. SUBSTANCE: method involves the use of antagonistically active strain Bacillus subtilis 3H (GISK N 248) producing neutral protease. Strain is cultured at sowing dose (3-5) x 109 cells/ml, medium saturation with oxygen - at the range 70-90%. Cultural fluid is separated from biomass by microfiltration followed by sterilizing filtration of the end enzyme preparation. Obtained biomass is used for preparing the preparation-probiotic. Proteolytic activity of cultural fluid is 46 ± 6 PU/g and that of ready concentrate - 50 ± 5 PU/g. Invention can be used for production of bacterial and enzyme preparations. EFFECT: improved method of preparing. 1 tbl
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