METHOD OF DETERMINING NITRATE-REDUCTION CAPACITY OF BIOLOGICAL FLUID Russian patent published in 1998 - IPC

FIELD: clinical biochemistry. SUBSTANCE: method includes sampling, dividing sample into test and reference portions, adding sodium nitrate to the test portion, incubation, adding reagents to the two portions, and determining nitrite content by reference to calibration curve and subsequently comparing data from the test and reference portions of sample. As reagent, crystalline sodium thiosulfate, ferric sulfate, and sodium ascorbate are used, and reaction mixtures obtained are frozen under liquid nitrogen. To determine nitrite concentration, EPR spectroscopy is involved. EFFECT: increased accuracy and reduced determination time.