FIELD: microbiology. SUBSTANCE: method involves dilution of biopreparation in buffer solution, treatment of bacterial suspension through cellular envelope and determination of fluorescence intensity difference of ethidium bromide in buffer solution and in bacterial suspension after its treatment. Total bacterium amount is determined by difference value. Method ensures to determine amount of living cells in biopreparation. EFFECT: improved method of quantitative determination. 2 cl, 2 tbl
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