METHOD OF MONITORING THE STAGE OF CULTIVATION IN THE TECHNOLOGY OF THE PLAGUE VACCINE Russian patent published in 2019 - IPC C12Q1/02 A61K39/02 C12N1/20 C12R1/01 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and can be used to monitor the stage of submerged cell cultivation in plague vaccine technology. Method of monitoring the stage of cultivation in the technology of live plague vaccine provides for a test 5-minute exposure of sodium dodecyl sulfate to the plague microbe cells, followed by photometric registration of the lytic effect and determination of the cell resistance index. In this case, the determination of the resistance index and the assessment of the functional state of the cells are carried out starting from 12 hours every 3 hours using special control intervals of resistance index 3.2 ± 1.20; 5.1 ± 1.11; 7.4 ± 1.05; 9.2 ± 0.99; 10.8 ± 0.90; 11.7 ± 0.84.

EFFECT: invention makes it possible to observe the change in the functional state of the culture during cultivation, to characterize the population according to its physiological maturity and usefulness for further processing and to improve the accuracy of the plague microbe cultivation process.

1 cl, 1 dwg, 3 ex, 3 tbl