METHOD OF PRODUCING TAXOL AND TAXONES FROM EMBRYO PLANT CULTURE OF TAXUS GENUS Russian patent published in 1998 - IPC

FIELD: biotechnology. SUBSTANCE: method involves using zygote embryos from yew seeds of species Taxus cuspidata as explants. Callus tissue is cultured up to formation of somatic embryos and then to embryogenic callus preparing. The latter is passaged in liquid nutrient medium and cultured on Hamburg medium mB₅ containing 2-4 ppm 2,4-D (2,4-dichlorophenoxyacetic acid) for cellular mass increase. Then culturing is continued on Murasige-Skoog Ms or Hamburg mB₅ medium containing 1-2 ppm NAA (α--naphthylacetic acid) that stimulates the yield of the end product. The end product is isolated by extraction from somatic embryos at stage of callus culturing or from embryogenic callus at stage of disinfected embryos culturing. The end product can be isolated from cellular suspension mass and cultural fluid. EFFECT: increased yield of taxol and taxones. 7 cl, 7 dwg, 3 tbl, 11 ex