FIELD: biotechnology, biochemistry.
SUBSTANCE: invention relates to extracts prepared from vegetable somatic embryos for the cell-free translation system and/or the coupled transcription-translation system. Method involves preparing embryonic callus from the primary material and the embryonic suspension culture. After induction of the secondary somatic embryogenesis extract is prepared from somatic embryos. Based on the extract the diagnostic system is developed for detection of biologically active compounds. Invention provides overcoming the species limitations and strain specificity and to attain the high effectiveness of the cell-free translation system and the coupled transcription-translation system also.
EFFECT: improved preparing method, valuable biological and biochemical properties of system.
49 cl, 5 dwg, 2 tbl, 9 ex
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Authors
Dates
2005-09-27—Published
2003-10-09—Filed