METHOD OF DETERMINATION OF ANTIPLATELET CATIONE-PROTEIN ACTIVITY OF MICROORGANISMS Russian patent published in 1998 - IPC

FIELD: microbiology. SUBSTANCE: method involves growing the examined microorganism culture on liquid nutrient medium, treatment with chloroform, supernatant separation, its incubation with platelet cation protein. Test-culture Bacillus subtilis GISK N 010011 is added to control and experimental samples. Obtained mixtures are incubated, sown on beef-extract agar and platings are incubated again. Then antiplatelet catione-protein activity of examined culture is calculated by the formula: x 100% where N_o is colony amount in experiment; is colony amount in control-1; is colony amount in control-2. Method ensures to detect and study new property of bacterial pathogens (antiplatelet cation-protein activity). EFFECT: increased precision of diagnosis, prognosis of sicknesses with microbe etiology and optimal therapy. 2 tbl, 2 ex