FIELD: microbiology. SUBSTANCE: method can be used for predicting reconvalescent germ carrying, for microbiological monitoring of anthropogenous environmental pollution, disbiosis diagnostics, and for studying effect of drugs on persistent characteristics of microorganisms. For that, culture under examination is grown in liquid nutrient medium, supernatant is separated, incubated with lysozyme, whereupon resultant mixture is added to suspension of test culture Micrococcus lysodeikticus preliminarily treated with Trilon B, and optical density of combined mixture is measured. Extent of lysis of Micrococcus test culture suspension is calculated by the aid of formula. EFFECT: enhanced efficiency of determining antilysozyme activity of microorganisms due to accelerated and more accurate evaluation technique.
Authors
Dates
1999-02-10—Published
1997-01-27—Filed