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2 156 807

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IPC

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Application

99117578/13, 1999-08-16

(24)

Start date

1999-08-16

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Actual filing date

1999-08-16

(45)

Published

2000-09-27

(72)

Inventor

Bukharin O.V.Valyshev A.V.Kharitonova T.V.Chernova O.L.Kirgizova S.B.

(73)

Holder

Institut Kletochnogo I Vnutrikletochnogo Simbioza Uro Ran

METHOD OF DETERMINATION OF ANTILACTOFERRIN ACTIVITY IN MICROORGANISMS Russian patent published in 2000 - IPC

Abstract RU 2156807 C2

FIELD: microbiology. SUBSTANCE: method involves culturing analyzed culture on liquid nutrient medium and supernatant from cultures grown in the presence of lactoferrin is obtained. In parallel two control specimens are prepared, incubated and test-culture of M. luteus GISK N 211001 is added to experimental and control specimens. Optical density of specimens is measured and antilactoferrin activity of studied culture is calculated by optical density value change. Method ensures to detect the novel property of microorganisms, antilactoferrin activity. Proposed method promotes to select rational antibacterial therapy, study persistence mechanism in microorganisms and formation of germ carrying. Invention can be used in laboratories of science-research institutes and bacteriological laboratories of clinics studying the assay of persistence properties of microorganisms (in experiment) and for study of their etiological significance in pathological processes (in clinics). EFFECT: improved method of assay. 1 tbl, 2 ex

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RU 2 156 807 C2

Authors

Bukharin O.V.

Valyshev A.V.

Kharitonova T.V.

Chernova O.L.

Kirgizova S.B.

Dates

2000-09-27—Published

1999-08-16—Filed

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