FIELD: immunology, oncology. SUBSTANCE: method involves adsorption of antigen on walls of plastic plate wells. Then biological fluid to be analyzed is incubated with adsorbed antigen and antibodies bound with antigen are developed with anti-antibodies labeled with an enzyme. Conjugate of acetic acid fluoromethylbenzanthryl with human serum albumin is used as an antigen at mass ratio 1:5.4, respectively, at concentration 2 mcg/ml. Method ensures to decrease nonspecific antibody binding in analyzed biological fluid with protein-carrier. EFFECT: simplified method, decreased antigen consumption. 2 dwg, 1 tbl
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Authors
Dates
1999-01-10—Published
1994-06-01—Filed