METHOD OF IMMUNOENZYMATIC ANALYSIS OF ANTIBODIES RAISED TO CHEMICAL CARCINOGENS OF POLYCYCLIC AROMATIC HYDROCARBON GROUP Russian patent published in 1999 - IPC

Abstract RU 2124731 C1

FIELD: immunology, oncology. SUBSTANCE: method involves adsorption of antigen on walls of plastic plate wells. Then biological fluid to be analyzed is incubated with adsorbed antigen and antibodies bound with antigen are developed with anti-antibodies labeled with an enzyme. Conjugate of acetic acid fluoromethylbenzanthryl with human serum albumin is used as an antigen at mass ratio 1:5.4, respectively, at concentration 2 mcg/ml. Method ensures to decrease nonspecific antibody binding in analyzed biological fluid with protein-carrier. EFFECT: simplified method, decreased antigen consumption. 2 dwg, 1 tbl

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RU 2 124 731 C1

Authors

Glushkov Andrej Nikolaevich

Klimov Igor' Aleksandrovich

Anosova Tat'Jana Petrovna

Kostjanko Mikhail Vladimirovich

Dates

1999-01-10Published

1994-06-01Filed