METHOD OF PREPARING AUTOCHTHON MICROORGANISM STRAINS BANK FOR RECOVERY OF HUMAN INTESTINE MICROBIOCENOSIS Russian patent published in 1999 - IPC

Abstract RU 2126043 C1

FIELD: medicine, microbiology. SUBSTANCE: method involves selection of feces samples from a single organism from its clinically health state beginning from 7-15 postnatal life and during spanlife by analysis once per a year. Autostrains of normal intestine microflora are isolated from samples and identified. Biomass of each bacterium species is produced separately on selective nutrient media to titer 103-109 cells/ml, not less. Obtained biomasses are combined, stabilizing composition is added and a mixture is divided for samples. Each sample is preserved and stored for human spanlife under periodical biotiter monitoring. At infant age (less 1 year) normal intestine microflora has the following bifidobacteria of species: Bifidobacterium bifidum, Bifidobacterium brevis, Bifidobacterium infantis and lactobacteria of species Lactobacillus acidophilus, Lactobacillus fermenti. At age above 1 year normal species of intestine microflora are mainly Bifidobacterium longum, Bifidobacterium adolescents, lactobacteria of species Lactobacillus acidophilus, Lactobacillus fermenti, Lactobacillus plantarum, strains of bacteria Escherichia coli and lactic acid streptococci of species mainly Streptococcus faecium, Streptococcum faecalis, Streptococcus avium, Streptococcus salivarius and Streptococcus bovis. During storage after biotiter control samples of bacterium autostrains isolated from normal human intestine microflora taken off at different spanlife are combined at an equal ratios. Administration of microorganism autostrains bank samples in human intestine ensures to effect on different links of damaged intestine bacteriocenosis simultaneously by means of using the more complete spectrum of normal intestine microflora. Invention can be used for prophylaxis and treatment of patients with intestine disbacteriosis in human. EFFECT: improved method of bank preparing, enhanced effectiveness of treatment. 7 cl, 6 ex

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RU 2 126 043 C1

Authors

Khachatrjan Ashot Papikovich

Khachatrjan Robert Grishaevich

Dates

1999-02-10Published

1997-07-29Filed