FIELD: medicine. SUBSTANCE: method involves taking saliva samples in morning time under empty stomach condition. The collected saliva is centrifuged during 10 min at 3000 revolutions per 1 min. Sample is prepared from transparent centrifuged saliva layer by diluting it with distilled water 1: 100. The sample under test and reference samples of the main standard solution are examined by applying flame photometry method with flame kept at 1200 C. Velocity of sample delivery to flame zone depends on aperture size and device sensitivity. Potassium (K) and sodium (Na) contents are determined in sample under test from formula C0= ((E0- Emin)/(Emax-Emin) x (Cmax-Cmin)+Cmin) x 100, where C0 is K- or Na- concentration in mg% in the sample under test, E0 is test sample extinction, Emin is minimum standard extinction most close to that of the sample under test, Emax is the maximum standard extinction most close to that of the sample under test, Cmax is the K- or Na- concentration in mg% corresponding to the minimum extinction. SU system recalculation coefficient from mg% to mmole/l is equal to 0.256 for potassium and 0.435 for sodium. Examination results are to be expressed in mmole/l in this case. K contents being less than 29.5 mmole/l and Na contents being less than 22.6 mmole/l, microvascular stenocardia case is diagnosed in ischemia patients. EFFECT: enhanced accuracy of diagnosis; simplified cheap method. 2 tbl
Authors
Dates
1999-07-20—Published
1998-01-20—Filed