METHOD OF PREPARING TRANSPLANT "BIO-MATRIX IMPLANT-1" FOR STOMATOLOGY Russian patent published in 1999 - IPC

FIELD: medicine, stomatology. SUBSTANCE: method involves the preliminary bone sawing cut for blocks (size is 3 x 2 x 1 cm), making the through holes (size from 0.1 to 10 mm) in each block plane (at leasts one hole per cm²). Blocks are washed out, treated with the enzyme mixture consisting of 0.1-1% trypsin solution and 0.125-0.3% papain solution taken at the ratio 1: 1 and then with the mixture of 1% solution of hydrogen peroxide and 1% ethanol solution taken at the ratio 1:1. Then blocks are washed out and each block is filled with material consisting of di- and/or trivalent metal salts, collagen, alkyl-, carboxyalkyl- or hydroxyalkyl-derivatives of cellulose, sulfated glycoseaminoglycanes and water. Then the each osseous block is frozen, lyophilized and sterilized by irradiation at the dose 2.5 Mgrad. EFFECT: increased degree of bone purification from fibrillar protein collagen, glycoproteins and glycolipids that are the specific indices of the tissue antigenicity. 1 ex