FIELD: medicine, biochemistry. SUBSTANCE: tissue is milled, treated by ultrasonic oscillation, hydrolyzed with enzymes mixture, boiled for 5-30 min, centrifuged at the rate from 3000 to 45000 rev/min and dialyzed for 5-6 days. After repeated centrifugation glycoseaminoglycans are precipitated with ethyl acetate solutions of alkaline and/or alkaline-earth metals. EFFECT: high content of glycoseaminoglycans in the end product, absence of proteins and other impurities. 1 ex
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Authors
Dates
1999-05-10—Published
1998-09-17—Filed