MODIFIED PROTEASE OF THERMOLYSIN-LIKE NEUTRAL METALLOPROTEASE, METHOD OF SYNTHESIS OF METHYL ETHER OF BENZYLOXYCARBONYL--α--L-ASPARTYL-L-PHENYLALANINE AND METHOD OF SPLITTING OF METHYL ETHER OF BENZYLOXYCARBONYL- α-L-ASPARTYL-L-PHENYLALANINE Russian patent published in 1999 - IPC

FIELD: chemical industry. SUBSTANCE: modified protease of thermolysin- like neutral metalloprotease has amino acid sequence SEQ ID N:I in which 150-th residue of aspartic acid is replaced with tryptophan. Possible also may be replacement of 227-th residue of asparagine with histidine and/or 144-th residue of leucine with serine. Modified protease is capable of catalyzing of process of synthesis and splitting of methyl ether of benzyloxycarbonyl-α-L-aspartyl-L-phenylalamine. EFFECT: higher efficiency. 6 cl, 13 dwg, 1 tbl