METHOD FOR PREPARING ALPHA-L-ASPARTYL-L-PHENYLALANINE-BETA-ESTER AND METHOD FOR PREPARING ALPHA-L-ASPARTYL-L-PHENYLALANINE-ALPHA-METHYL ESTER Russian patent published in 2007 - IPC C12P21/02 

FIELD: biotechnology, food processing industry, derivatives of amino acids.

SUBSTANCE: invention proposes a method for enzymatic preparing α-L-aspartyl-L-phenylalanine-β-ester (α-L-(β-o-substituted aspartyl)-L-phenylalanine) from L-aspartic acid α,β-diester and L-phenylalanine at temperature 5-40⁰C and pH 7-10 for period sufficient for formation of α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid α,β-diester and L-phenylalanine. Proposed method is used in a method for preparing α-L-aspartyl-L-phenylalanine-α-methyl ester that comprises a step for synthesis of α-L-aspartyl-L-phenylalanine-β-methyl ester and step for conversion of indicates ester to α-L-aspartyl-L-phenylalanine-α-methyl ester. Using this invention provides synthesis of α-L-aspartyl-L-phenylalanine-β-ester followed by preparing α-L-aspartyl-L-phenylalanine-α-methyl ester by simplified and inexpensive scheme and with enhanced yield of the end product.

EFFECT: improved method of synthesis.

5 cl, 1 dwg, 4 tbl, 12 ex