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2 138 291

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IPC

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Application

98105568/13, 1998-03-30

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Start date

1998-03-30

(22)

Actual filing date

1998-03-30

(45)

Published

1999-09-27

(72)

Inventor

Balyshev V.M.Bashaev V.V.Zhesterev V.I.Vishnjakov I.F.Gorshkova T.F.Shchetnikova L.A.Isakova N.B.Jurkov S.G.

(73)

Holder

Vserossijskij Nauchno-Issledovatel'Skij Institut Veterinarnoj Virusologii I Mikrobiologii

METHOD OF PREPARING VACCINE AGAINST SMALLPOX IN SHEEP Russian patent published in 1999 - IPC

Abstract RU 2138291 C1

FIELD: veterinary science, vaccines. SUBSTANCE: invention relates to preparing live cultural vaccines against smallpox in sheep. Method involves preparing sowing sets of transplantable sheep kidney cells (sublines PO-VNIIVViM) in roller flasks. Formed monolayer is infected with sowing virus at the optimal dose. Infected culture is incubated under roller conditions for 5-6 days and the supporting medium is changed in every 2-3 days of culturing. Cultures at the degree of the damage by 75-80% are frozen at -40 C and thawed at the room temperature. Obtained virus-containing raw is mixed with protective medium of the definite composition at their volume ratio = 1:1 followed by lyophilic drying up to the content of moisture 4%, not above. Invention provides preparing the standard, harmless, highly immune and stable at storage vaccine against smallpox in sheep. EFFECT: improved quality and properties of vaccine, highly productive technology of preparing. 1 tbl, 4 ex

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RU 2 138 291 C1

Authors

Balyshev V.M.

Bashaev V.V.

Zhesterev V.I.

Vishnjakov I.F.

Gorshkova T.F.

Shchetnikova L.A.

Isakova N.B.

Jurkov S.G.

Dates

1999-09-27—Published

1998-03-30—Filed