PLASMID 40NaGal, DETERMINING SYNTHESIS OF α-N-ACETYLHALACTOSAMINIDASE OF α-AlNaGal, STRAIN E.coli Rosetta(DE3)/40NaGal - PRODUCER OF CHIMERIC PROTEIN, WHICH INCLUDES AMINOACID SEQUENCE OF RECOMBINANT α-N-ACETYLHALACTOSAMINIDASE OF α-AlNaGal, AND METHOD OF ITS OBTAINING Russian patent published in 2014 - IPC C12N15/00 C12N1/21 

FIELD: chemistry.

SUBSTANCE: invention represents the plasmid 40NaGal, which determines synthesis of α-acetylhalactosaminidase α-AlNaGal, which includes a NcoI/SalI-fragment of the plasmid pET-40b(+) (Novagen) and a fragment of DNA with a size of 1299 base pairs, which contains a chimeric gene, consisting of a structural part of the gene α-AlNaGal, adapted by the N-end for the expression in cells of E.coli, and nucleotides, coding a specific sequence for the protease TEV. The invention also relates to strain of E.coli Rosetta(DE3), transformed by the claimed plasmid, - producent of a chimeric protein, which includes an aminoacid sequence of recombinant α-N-acetylhalactosaminidase α-AlNaGal. Also claimed is a method of obtaining recombinant α-N-acetylhalactosaminidase α-AlNaGal with the application of strain according to the invention.

EFFECT: invention makes it possible to obtain α-N-acetylhalactosaminidase with a high efficiency degree.

3 cl, 1 dwg, 3 ex