METHOD OF DETERMINING LEUKOCYTE PHAGOCYTIC ACTIVITY Russian patent published in 1999 - IPC

FIELD: medicine, pathophysiology, immunology. SUBSTANCE: heparinized blood (5-6 ml) is taken in patient and leukocytes are isolated by settling and centrifugation. Leukocyte suspension is prepared at final concentration 12-25000 cells in 1 mcl. Baker's yeast preliminary labeled with trypan blue to obtain intensely blue color is used as the phagocytosis object. Yeast concentration is brought about to 3-5 or more cells/1 leukocyte. Leukocyte suspension is incubated with 0.4 ml of yeast cells suspension at 37 C for 40 min and amount of phagocytic cells are counted in Goryaev's chamber. Method ensures to count simultaneously absolute and per cent values of phagocytic indices in 1 mcl of analyzed suspension, decrease procedures number, improve the differentiation of phagocytosis object from subcellular phagocyte particles, exclude the necessary of microbiological technique of preparing the phagocytosis object and the separate additional counting the total (absolute) amount of leukocytes. EFFECT: simplified technology, decreased time consumption, increased precision of assay. 1 ex