FIELD: cosmetology and dermatology. SUBSTANCE: culture medium for cells contains ecdisteroid, ecdisteroid derivative or containing its vegetable and/or animal extract - agent or regulation of cells differentiation. Culture medium may be preferably used in method for mass cultivation of keratinocytes. The method provides for perfusion of nutrient medium and its sufficient quantity fro growth and differentiation even when biomass is in considerable quantities. The agent of regulation of cells differential makes it possible to improve quality of cosmetic and dermatological compositions. EFFECT: higher efficiency. 24 cl, 1 tbl, 12 ex
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