FIELD: hematology. SUBSTANCE: crude 0.1 to 20% hemoglobin solution is passed continuously through anionite or cationite chromatographic column until nearly complete saturation of column with hemoglobins and components with higher affinity, and further passage of solution proceeds to achieve supercharge of column, after which hemoglobins are replaced. Second chromatographic step is anticipated. EFFECT: extended choice of methods for commercial-scale hemoglobin purification. 10 cl, 16 dwg, 9 tbl, 18 ex
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Authors
Dates
2000-02-27—Published
1994-09-20—Filed