FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology. Disclosed are a method of purifying a composition containing low IET antibodies (embodiments); composition containing IET antibodies from 3.0 to 8.0 and having less tendency to formation of antibody aggregates, and method of its production, method for preparing pharmaceutical composition. Method of purifying the composition involves removing impurities from the composition by eluting IET antibodies from 3.0 to 8.0 from an anion-exchange resin in a binding/elution mode using a solution for elution with a higher salt concentration or by treating the composition in acidic conditions with further neutralization. Composition contains antibodies with IET from 3.0 to 8.0 with content of antibody aggregates of 3 % or less.
EFFECT: inventions provide effective removal of impurities contained in the culture medium of the antibodies.
19 cl, 15 tbl, 10 ex
| Title | Year | Author | Number |
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