IMPROVING IMMUNOGLOBULIN AFFINITY CHROMATOGRAPHY BY THE APPLICATION OF PRE-CAPTURE FLOCULATION Russian patent published in 2023 - IPC C07K1/22 C07K1/34 C07K1/36 

FIELD: pharmaceutical industry.

SUBSTANCE: method for carrying out affinity chromatography with the capture of immunoglobulin from the culture liquid includes the following steps: adding a water-soluble organic polymer that causes flocculation to the culture liquid, depth filtration and affinity chromatography of the filtrate of the resulting mixture, while the immunoglobulin is associated with the medium for affinity chromatography, then the medium for affinity chromatography is washed using a wash buffer of pH 5–9 and an ionic strength of 0.1–5 mol/l and eluting the immunoglobulin from the affinity chromatography resin using an elution buffer of pH 2.5–4.5, with a water-soluble organic polymer being a poly(diallyldimethylammonium chloride) with a molecular weight of 10-10,000 kDa, which is added at a final concentration of 0.01–1.0 w/v.%.

EFFECT: elimination of formation of a precipitate in affinity chromatography.

24 cl, 5 dwg, 19 tbl, 16 ex