METHOD OF OBTAINING PREPARATION OF ANTIBODY AGAINST IL-18 OR ITS ANTIGEN-BINDING PART (VERSIONS) Russian patent published in 2014 - IPC C07K16/00 C07K16/24 C07K1/18 C07K1/20 A61K39/395 

FIELD: medicine, pharmaceutics.

SUBSTANCE: group of inventions relates to methods of obtaining preparation of antibody against IL-18 or its antigen-binding part with reduced content of host cell protein (HCP) from sample of mixture, containing antibody against IL-18 or its antigen-binding part, and, at least, one HCP. Method includes reduction of mixture sample pH from 3.0 to approximately 4.0, bringing pH from 4.5 to approximately 5.5 and conductivity to 9±0.5 mS/cm, application of sample on cation-exchange resin, carrying out chromatography of hydrophobic interaction and sample collection. Version of method includes realisation of anion-exchange chromatography before the stage of cation-exchange chromatography. Version of method also includes sample filtration before anion-exchange chromatography.

EFFECT: group of inventions makes it possible to obtain preparation of antibody with increased output and purity Output constitutes 96±4%, purity 99,51±0,26%.

28 cl, 2 dwg, 9 tbl, 2 ex