METHOD FOR EXAMINING CELLULAR BLOOD COMPOSITION USING A SMEAR Russian patent published in 2000 - IPC

FIELD: medicine. SUBSTANCE: method involves counting leukocyte formula, selecting normal and pathological forms of erythrocytes, recording blood platelets distribution in sizes and determining the number of erythrocytes, leukocytes and blood platelets on the smear area in doing the following operations. The preparation is scanned and a series of frames are recorded as input into computer. The frames have images of microscope vision fields. The frames are segmented, the cell numbers of each type (erythrocytes, leukocytes and blood platelets) are counted, their quantitative relationship is determined, the selected objects are measured, blood platelets size distribution is determined. Erythrocyte and leukocyte cells image recognition (classification) is carried out. Normal and pathologic forms of erythrocytes are selected, their ratios are determined. Leukocyte formula is determined. The number of erythrocytes, leukocytes and blood platelets in blood volume is determined using the method described for the case of smears prepared as monolayers from a given blood volume. The results are recalculated by transforming the area-based results into the spatial ones. EFFECT: enhanced accuracy in examining cell composition of blood smears.