FIELD: medicine. SUBSTANCE: method involves differentiating glycosamineglycanes as belonging to some classes. Standard thickness histologic slices are stained in advance with alcian blue at pH from 1.0 to 4.0 with NaCl solution of 0.9% concentration and magnesium chloride of 0.1 to 1.0 molar solution added as electrolytes under enzymatic control and methylation. EFFECT: enhanced accuracy in differentiating glycosamineglycane classes in brain tissue; simplified and low cost method.
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