METHOD FOR OBTAINING PHARMACEUTICAL SULFATED GLYCOAMINOGLYCANS FROM BIOLOGICAL TISSUES Russian patent published in 2021 - IPC C08B37/08 C08B37/10 C12P19/04 

FIELD: pharmaceutical industry.

SUBSTANCE: invention relates to the pharmaceutical industry and can be used to obtain sulfated glycoaminoglycans from biological tissues. Raw materials are cleaned, disinfected with sodium hypochlorite, washed with water, and crushed. Alkaline hydrolysis is carried out until the precipitate is completely dissolved. The resulting mixture is neutralized by acidification to pH 5.5-6.5. Enzymatic hydrolysis is carried out using a complex of enzymes papain, trypsin and chymotrypsin at a temperature of 30-40°C. Enzymes are deactivated by heating to a temperature of 65-70°C and the hot solution is sequentially filtered on membranes with a pore size on the final membrane of no more than 0.2 mcm. Ultrafiltration of the enzymatic hydrolyzate is carried out on a membrane with a retention limit of 2.5 kDa. The product retained on the membrane is washed with sodium chloride solution and concentrated to obtain a solution of sulfated glycosaminoglycans. A solution of sulfated glycosaminoglycans is filtered through a membrane with a pore size of 0.2 mcm and used as a finished product or sent to obtain a dry finished product.

EFFECT: invention makes it possible to obtain pharmaceutical compositions with a given percentage of specific sulfated glycosaminoglycans, to use a wider range of low-value by-products from which chondroitin sulfates can be isolated, to increase the yield of the target product, to more fully isolate accompanying aGAGs (dermatan sulfate, keratan sulfate), and also to increase the purity of the final product.

4 cl, 2 tbl, 4 ex