FIELD: molecular biology, genetic engineering. SUBSTANCE: invention relates to preparing the novel, original, artificially produced polypeptide consisting of product of expression of gene env HTLV-I fragment fused with E. coli beta-galactosidase and binding antibodies raised to product of gene env HTLV-I, DNA ATLE 495 fragment, strain of E. coli HB101 transformed with recombinant plasmid pATLE 495 containing DNA ATLE 495 fragment. Invention ensures to obtain 200-350 mg of hybrid protein from 1 l suspension of E. coli cells. This hybrid protein has the specific polypeptide sequence encoded by region env of genome HTLV-I and can be used for serodiagnosis of HTLV-I carrying out. EFFECT: high valuable biological properties. 4 cl, 4 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA PESG, METHOD OF PREPARING RECOMBINANT PLASMID DNA PESG AND STRAIN OF BACTERIUM ESCHERICHIA COLI CONTAINING RECOMBINANT PLASMID DNA PESG USED FOR PREPARING THE HYBRID PROTEIN CONSISTING OF 485 AMINO ACIDS SHOWING ANTIGENIC PROPERTIES OF HUMAN VIRUS T-CELLULAR LEUKOSIS OF THE FIRST TYPE |
1994 |
- Garaev M.M.
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|
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1998 |
- Bobkov A.F.
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- Bondarenko V.O.
|
RU2149876C1 |
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1993 |
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|
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0 |
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|
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1990 |
- Kiprijanov S.M.
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- Serpinskij O.I.
|
SU1766070A1 |
| POLYPEPTIDE DESIGNED FOR ANTIBODY ASSAY TO HUMAN IMMUNODEFICIENCY VIRUS OF THE SECOND TYPE, DNA FRAGMENT HE206 ENCODING POLYPEPTIDE E206, RECOMBINANT PLASMID DNA PHE206 ENCODING POLYPEPTIDE E206, STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE E206 |
1992 |
- Zajtsev I.Z.
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|
RU2043412C1 |
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1992 |
- Zajtsev I.Z.
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|
RU2043414C1 |
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1995 |
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1993 |
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