FIELD: biotechnology, genetic engineering. SUBSTANCE: invention relates to methods of detection of human virus T-cellular leukosis the first type (HTLV-1). Method involves preparing recombinant plasmid DNA providing high level of hybrid gag-protein HTLV-1 production showing antigenic property and preparing the strain-producer of the indicated protein. Plasmid pGdN is obtained by fusion of plasmid pVR-290 fragment encoding E. coli β-galactosidase, two polylinkers, start replication region and gene determining the resistance to ampicillin with plasmid MT2 fragment containing the sequence of gag-protein HTLV-1. E. coli cells were transformed with the obtained plasmid and the strain GKV/pGdN is selected. This strain produces hybrid protein containing virus-specific sequence of gene gag HTLV-1. After the strain GKV/pGdN culturing the yield of protein showing antigenic properties of HTLV-1 is 400-500 mg/1 l cell suspension. EFFECT: improved method of plasmid preparing, enhanced effectiveness of strain-producer. 4 cl, 3 dwg
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA PESG, METHOD OF PREPARING RECOMBINANT PLASMID DNA PESG AND STRAIN OF BACTERIUM ESCHERICHIA COLI CONTAINING RECOMBINANT PLASMID DNA PESG USED FOR PREPARING THE HYBRID PROTEIN CONSISTING OF 485 AMINO ACIDS SHOWING ANTIGENIC PROPERTIES OF HUMAN VIRUS T-CELLULAR LEUKOSIS OF THE FIRST TYPE |
1994 |
- Garaev M.M.
- Bobkov A.F.
- Sankov M.N.
|
RU2081172C1 |
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1998 |
- Bobkov A.F.
- Sankov M.N.
- Bondarenko V.O.
|
RU2149876C1 |
| RECOMBINANT PLASMIDE DNA P P1 DIRECTED SYNTHESIS OF FUSED PROTEIN SHOWING ANTIGEN PROPERTIES OF HUMAN IMMUNODEFICIENCY VIRUS I, METHOD OF ITS CONSTRUCTING AND A STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF FUSED PROTEIN SHOWING ANTIGEN PROPERTIES OF HUMAN IMMUNODEFICIENCY VIRUS I |
0 |
- Garaev Mansur Mukhamedovich
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|
SU1816797A1 |
| FRAGMENT OF GENE ENV HTLV-I, RECOMBINANT PLASMID DNA PATLE 495, STRAIN OF BACTERIUM ESCHERICHIA COLI HB101/PATLE 495 AS PRODUCER OF POLYPEPTIDE E 495 |
1997 |
- Bobkov A.F.
- Sankov M.N.
- Selimova L.M.
- Kazennova E.V.
|
RU2152955C2 |
| RECOMBINANT PLASMID DNA PBMC-GAG(A)MOD FOR EXPRESSION OF HUMAN IMMUNODEFICIENCY VIRUS PROTEIN GAG IN EUCARYOTIC CELLS |
2003 |
- Kozlov A.P.
- Klimov N.A.
- Masharskij A.Eh.
- Poljakova M.S.
- Romanovich A.Eh.
- Dukhovlinov I.V.
- Murashev B.V.
|
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0 |
- Garaev Mansur Mukhamedovich
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- Bobkova Marina Ridovna
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|
SU1708848A1 |
| RECOMBINANT PLASMID DNA PUL 44 HCMV DETERMINING EXPRESSION OF HUMAN CYTOMEGALOVIRUS GENE UL 44 FRAGMENT ENCODING IMMUNODOMINANT SITE OF DNA-BINDING PROTEIN P 52 (ICP 36) IN ESCHERICHIA COLI BACTERIUM CELLS |
1998 |
- Susloparov M.A.
- Susloparov I.M.
- Pljasunov I.V.
|
RU2151800C1 |
| RECOMBINANT PLASMID DNA PU11HHV6 PROVIDING EXPRESSION OF GENE U11 FRAGMENT OF HUMAN HERPES VIRUS OF 6 TYPE ENCODING IMMUNODOMINANT MOIETY OF PROTEIN TEGUMENT P100 IN CELLS OF BACTERIUM ESCHERICHIA COLI |
2000 |
- Susloparov M.A.
- Bakhtina M.M.
- Susloparov I.M.
- Makhova N.M.
- Pljasunov I.V.
- Lokteva L.A.
|
RU2189393C2 |
| RECOMBINANT PLASMID DNA PQ-F35 ENCODING FUSED POLYPEPTIDE F35 SHOWING ANTIGENIC AND IMMUNOGENIC PROPERTIES OF MARBURG VIRUS PROTEIN VP 35, METHOD OF ITS PREPARING AND STRAIN OF BACTERIUM ESCHERICHIA COLI - SUPERPRODUCER OF RECOMBINANT POLYPEPTIDE F35 |
1998 |
- Sorokin A.V.
- Razumov I.A.
- Kazachinskaja E.I.
- Kachko A.V.
- Ivanova A.V.
- Mishin V.P.
- Bukreev A.A.
- Belanov E.F.
- Loktev V.B.
- Netesov S.V.
|
RU2144565C1 |
| RECOMBINANT PLASMID DNA PURVGF DETERMINING SYNTHESIS OF SMALLPOX VIRUS STRAIN L-IVP GROWTH FACTOR TOGETHER WITH ESCHERICHIA COLI BETA-GALACTOSIDASE AND STRAIN OF BACTERIUM ESCHERICHIA COLI CONTAINING RECOMBINANT PLASMID DNA PURVGF - A PRODUCER OF SMALLPOX VIRUS STRAIN L-IVP GROWTH FACTOR |
1993 |
- Petrov V.S.
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- Raspopin V.V.
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- Krendel'Shchikov A.V.
- Malygin Eh.G.
|
RU2089612C1 |
