FIELD: molecular biology, biotechnology, genetic engineering, immunology. SUBSTANCE: invention relates to the novel artificial polypeptide consisting of product expression of fragment HTLV-1 of gene gag fused with E. coli beta-galactosidase and showing response to product of gene gag HTLV-I/II, and to fragment ATLG 579 of DNA, strain of E.coli HB 101 transformed with recombinant plasmid pATLG 579 containing fragment ATLG 579 of DNA. The source of HTLV-I/II-gag-specific nucleotide sequences - mononuclear cells of HTLV-infected donor. DNA fragment corresponding to region of gene gag HTLV-I/II and encoding region of genome with coordinates 1253-1952 nucleotide pairs was amplified in polymerase chain reaction. Recombinant plasmid pATLG 579 is obtained by ligation of DNA-amplified fragment (size is 699 nucleotide pairs) using T4 phage DNA-ligase. This fragment was isolated from agarose gel and hydrolyzed with enzymes Bam HI and ClaI. Ligation was carried out with linearized DNA of plasmid vector pUR 290. After incubation competent E. coli cells are transformed with hybrid DNA followed by selection of transformed cell colonies. Invention ensures to obtain 200-350 mg of protein/1 l of cellular suspension and this protein shows antigenic properties of HTLV-I/II. Invention can be used serodiagnosis of HTLV-I/II carrying out. EFFECT: new polypeptide, improved method of preparing. 4 cl, 6 ex
