FIELD: chemistry; biochemistry.
SUBSTANCE: present invention pertains to biotechnology, particularly to methods of obtaining vaccine preparations using genetic engineering and immunology methods. Proposal is given of pBMC-gp140(A)-hum expression plasma DNA, containing the gp140(A)-hum artificial gene, for expressing the Gp140(A)-hum protein in eukaryotic organism cells, which allows for 6-8 fold increase of output of the synthetic Gp140(A)-hum protein compared to the prototype. The invention can be used in medicine and related fields for increasing expression of proteins, particularly the gp140 gene encoded protein Gp140 or its fragments in eukaryotic organism cells.
EFFECT: obtaining vaccine preparations using genetic engineering and immunology methods.
3 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| EXPRESSION PLASMID DNA pBMC - nef (A)-hum | 2006 |
|
RU2335540C1 |
| EXPRESSIVE PLASMID DNA p-BMC-gag (A)-HUM FOR EXPRESSION OF PROTEIN p55 HJV-1 IN EUKARYOTE CELLS | 2006 |
|
RU2345138C2 |
| RECOMBINANT PLASMID DNA pBMC-RT(A)-hum FOR EXPRESSING PROTEIN OF REVERSE TRANSCRIPTASE OF HUMAN IMMUNODEFICIENCY VIRUS | 2006 |
|
RU2355764C2 |
| RECOMBINANT PLASMID DNA PBMC-QP120(A)M-1 FOR IMMUNIZATION AGAINST HUMAN IMMUNODEFICIENCY VIRUS | 2003 |
|
RU2227161C1 |
| RECOMBINANT PLASMID DNA PBMC-GAG(A)MOD FOR EXPRESSION OF HUMAN IMMUNODEFICIENCY VIRUS PROTEIN GAG IN EUCARYOTIC CELLS | 2003 |
|
RU2241752C1 |
| RECOMBINANT PLASMID DNA PBMC-RT(A)M-1 USED FOR EXPRESSION OF HUMAN IMMUNODEFICIENCY VIRUS REVERSE TRANSCRIPTASE (RT)IN HIGHER EUCARYOTIC CELLS | 2003 |
|
RU2238977C1 |
| RECOMBINANT PLASMID DNA pBMC-nef(A)m-1 EXPRESSING PROTEIN NEF OF HUMAN IMMUNODEFICIENCY VIRUS TYPE I VIRUS IN EUCARYOTIC CELLS | 2003 |
|
RU2229518C1 |
| IMMUNOPROPHYLACTIC VACCINE AGAINST HIV BASED ON HIV-SPECIFIC ANTIBODIES | 2008 |
|
RU2505604C2 |
| CARTRIDGE GENETIC CONSTRUCT EXPRESSING TWO BIOLOGICALLY ACTIVE SIRNAS EFFECTIVELY ATTACKING TARGETS IN MRNA OF VPU AND ENV GENES OF HIV-1 SUBTYPE A IN RUSSIAN PATIENTS, AND ONE SIRNA AIMED AT CCR5 GENE MRNA | 2016 |
|
RU2630644C1 |
| COMPOSITIONS AND METHODS OF GENETIC MATERIAL DELIVERY | 1994 |
|
RU2174845C2 |
