FIELD: biochemistry, ecology. SUBSTANCE: invention relates to control of environment pollution. Method involves the measurement of the rate of enzymatic hydrolysis of acylthiocholine (substrate for choline esterase) in buffer medium in the absence and in the presence of sample to be analyzed using indicator for thiol group. 4,4'-Bis-(2-hydroxy-6,8-disulfo-1-naphthylazo)-phenyl disulfide tetrapotassium salt is used as indicator. The rate value is measured by tangent angle slope in kinetic curve initial site. Method provides elimination of dilution of analyzed colored extracts. EFFECT: decreased limit of assay and error of analysis of anticholine esterase compound. 3 cl, 4 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETERMINING BLOOD CHOLINESTERASE ACTIVITY | 1998 |
|
RU2153675C2 |
| RAPID METHOD OF DETECTING BUTYRYLCHOLINESTERASE INHIBITORS IN WATER AND AQUEOUS EXTRACTS | 2019 |
|
RU2704264C1 |
| CHOLINESTERASE INHIBITOR DIAGNOSTIC KIT | 2000 |
|
RU2182929C2 |
| OPTICAL BIOSENSOR OF IRREVERSIBLE CHOLINE ESTERASE IN ATMOSPHERE | 2000 |
|
RU2198394C2 |
| METHOD OF ANTICHOLINESTERASE SUBSTANCE ASSAY IN SOLUTIONS | 2007 |
|
RU2358014C2 |
| QUANTITATIVE SPECTROPHOTOMETRIC METHOD FOR DETERMINING AND IDENTIFYING ORTHO- CHLOROBENZILIDENEMALONIC ACID DINITRILES IN EXTRACTS | 2002 |
|
RU2207548C1 |
| METHOD FOR EXPRESS-EVALUATION OF SUBSTANCES ON SOLID SURFACES | 2002 |
|
RU2215284C2 |
| METHOD OF QUANTITATIVE DETERMINATION OF CYANIDES | 2002 |
|
RU2217745C2 |
| EXPRESS-METHOD FOR ASSAY OF MICROSCOPIC FUNGI, BACTERIA AND YEASTS | 2004 |
|
RU2276190C2 |
| METHOD AND DEVICE FOR DETERMINING CHOLINESTERASE ACTIVITY IN BLOOD | 0 |
|
SU1668949A1 |
