METHOD OF QUANTITATIVE ESTIMATION OF ALIPHATIC AMINO ACIDS Russian patent published in 2001 - IPC

FIELD: analytical chemistry, more particularly methods of quantitative estimation of aliphatic amino acids for inspecting quality of products manufactured by pharmaceutical industry and prepared by drugstores. SUBSTANCE: estimation is carried out by treating sample under study with 1% alcoholic solution of ninhydrin in buffer phosphate solution with pH from 6.4 to 7.6 in the presence of 1 mg of ascorbic acid. The resulting colored solution is diluted with water and optical density is measured by spectrophonometer using wave length of 568 nm in cell with layer thickness of 1 cm. EFFECT: higher sensitivity, simpler and cheaper estimation. 2 tbl