FIELD: analytical chemistry, more particularly methods of quantitative estimation of aliphatic amino acids for inspecting quality of products manufactured by pharmaceutical industry and prepared by drugstores. SUBSTANCE: estimation is carried out by treating sample under study with 1% alcoholic solution of ninhydrin in buffer phosphate solution with pH from 6.4 to 7.6 in the presence of 1 mg of ascorbic acid. The resulting colored solution is diluted with water and optical density is measured by spectrophonometer using wave length of 568 nm in cell with layer thickness of 1 cm. EFFECT: higher sensitivity, simpler and cheaper estimation. 2 tbl
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Authors
Dates
2001-05-20—Published
1999-08-03—Filed