METHOD OF QUANTITATIVE DETERMINATION OF REDUCING SUGARS Russian patent published in 2010 - IPC G01N33/15 G01N33/02 

FIELD: medicine.

SUBSTANCE: acid hydrolysis of 0.3 g of laminarid or laminarid granules with 15 ml of 10% sulfuric acid, volume of hydrolysate is brought to 50 ml. Volume of aliquot part of solution 10 ml is taken and neutralised first with sodium hydroxide to pH 9.7, then with solution of sulfuric acid to pH 3.5, after that with solution of sodium hydroxide to pH 8.9, pH of solutions is controlled in potentiometrically. Filtration is carried out, volume of filtrate is brought to 25 ml with water, 1 ml of obtained solution is treated with 0.2 ml of 1% of picric acid solution in presence of 3 ml of 20% of sodium carbonate solution with heating. Volume of solution is brought to 25 ml and optic density is measured at water background spectrophotometrically at wavelength 356 nm with simultaneous measurement in the same conditions of optic density of 0.2 ml of 1% of picric acid solution and of glucose solution. Content of reducing sugars (C,%) is calculated by formula: where a₁ is portion of glucose, in g; a₂ is portion of polysaccharide preparation, in g; D₁ is optic density of picric acid solution; D₂ is optic density of glucose solution; D₃ is optic density of tested solution; W is loss of glucose in mass during drying, in %; 5000 is coefficient which takes into account dilutions of solutions in analysis.

EFFECT: invention allows to increase accuracy and reproducibility of results.

2 ex, 4 tbl, 3 dwg