METHOD OF PREPARING VACCINE BCG-M FOR SPARING PRIMARY IMMUNIZATION Russian patent published in 2001 - IPC

FIELD: medicine. SUBSTANCE: bacterial mass of culture BCG-M grown in solid potato medium and then in liquid synthetic media is removed, filtered, washed out with drying medium, ground in shuttle-apparatus to obtain homogeneous mass, diluted with stabilizing agent to prepare suspension containing 50 mg of BCG/1 ml followed by centrifugation at 2 000 rev/min for 15-20 min. Then supernatant suspension is diluted again to the final concentration 0.5 mg BCG/1 ml. Vaccine is ampouled and lyophilized. EFFECT: enhanced viability in single vaccinating dose. 1 cl, 3 tbl, 1 ex