METHOD OF DEUTERIUM-FRUCTOSE PRODUCING Russian patent published in 2001 - IPC

FIELD: microbiological industry, biotechnology. SUBSTANCE: method involves culturing bacterial strain Methylobacillus flagellatum VKPM B-7823 in nutrient medium containing deuterium-methanol as the source of carbon, heavy water and mineral salts. Bacterial cells of the strain-producer adaptated for heavy water medium synthesize in these media deuterium-polyfructanes consisting of monomers of deuterium-fructose with impurity of deuterium-ribose (2%). The end substance, deuterium-fructose, is isolated as a dry matter after isolation of deuterium-polyfructanes from cultural broth and their hydrolysis. Method provides to obtain up to 100 mg/l of deuterium-fructose with the level of deuterium incorporation 89%, not less. EFFECT: improved method of producing. 1 ex