FIELD: analysis of mixtures and solutions for content of biological and/or chemical components. SUBSTANCE: process is related to detection of attachment of biological and/or chemical components of liquid or gas mixtures and solutions, predominantly of biological origin to materials binding these components on basis of biological, chemical or physical coupling. Binding materials are placed on surface or in the thick of sensor layer which alters its optical thickness as result of detected attachment. Layer is subjected to effect of light of various wave lengths, Signal attributed to interference on sensor layer is registered in reflected or passed light. According to first variant this signal is spectrum. In this case sensor layer has thickness over 10 μm and at least an order of magnitude greater than maximum registered wave length. Information on examined attachment is obtained from analysis of spectral shift of interference maxima or minima. In agreement with second variant light is let pass through scanned Fabry-Perot interferometer and registered signal in this case is dependence of intensity of resulting light on change of base of scanned Fabry-Perot interferometer in which maxima caused by correlation of spectral characteristics of interaction of light with sensor layer and interferometer are observed. In formation on detected attachment is obtained from shift of this dependence relative to value of base. Technical result of process consists in securing of independence of measurement results from uncontrolled variations of intensity of analyzed light both on the whole and across individual sections of spectrum and in individual regions of area of sensor layer and consequently in increased measurement accuracy and in raised authenticity of results, in rise of sensitivity and resolution with simultaneous reduction of number of required operations. EFFECT: reduced labor input and cost of process both in single-channel and multichannel variants including registration under real time mode. 95 cl, 2 dwg
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Authors
Dates
2002-04-20—Published
2000-05-11—Filed