METHOD OF VITAMIN D-BINDING PROTEIN (PROTEIN Gc) CLONING, METHOD OF CLONED MACROPHAGE ACTIVATION FACTOR PREPARING, MACROPHAGE ACTIVATION FACTOR (VERSIONS), ADJUVANT (VERSIONS), CLONED VITAMIN D- BINDING PROTEIN (Gc-PROTEIN), CLONED DOMAIN III OF VITAMIN D-BINDING PROTEIN (VERSIONS) Russian patent published in 2003 - IPC C12N15/10 C07K14/435 C12P21/02 

FIELD: molecular biology, protein engineering, medicine. SUBSTANCE: invention relates to strong factors of macrophages activation. Vitamin D₃ -binding protein (Gc-protein) and its small domain (about 1/5 of Gc-peptide) that is known as domain III also) is cloned using baculovirus vector. The cloned Gc-protein and cloned peptide domain III (Cd) are treated with immobilized β-galactosidase and sialidase and factors of activation of macrophages GcMAFc and CdMAF, respectively, are prepared. These cloned factors of activation of macrophages and GcMAF can be used as adjuvants in immunization and vaccination. Invention allows to treat sicknesses, for example, osteopetrosis. EFFECT: improved method of cloning and preparing, valuable medicinal properties. 11 cl, 11 dwg, 4 tbl