FIELD: biotechnology.
SUBSTANCE: invention refers to the area of biotechnology. A fusion protein that is expressed in a recombinant protein body-like assembly (RPBLA) in host eukaryotic cells and organisms is disclosed, said fusion protein containing two sequences linked together in which one sequence is a protein body-inducing sequence (PBIS) and the other is a biologically active polypeptide, said biologically active polypeptide being naturally found in a second cell type that is different from said eukaryotic host cell. Recombinant protein body-like assemblies (RPBLAs) that comprise a membrane-enclosed fusion protein are disclosed. Methods for preparing a vaccine or inoculum comprising an immunogenic effective amount of recombinant protein body-like assemblies (RPBLAs) that contain a recombinant fusion protein dissolved or dispersed in a pharmaceutically acceptable diluent are disclosed, too.
EFFECT: disclosure of membrane-enclosed fusion protein.
38 cl, 8 dwg, 27 ex
