METHOD OF PROPOLIS FRACTIONATION Russian patent published in 2003 - IPC

FIELD: apiculture. SUBSTANCE: method involves ground propolis or canvases covered with propolis as the parent material. Extraction is carried out for two stages. In the first stage extraction is carried out with condensed carbon dioxide under pressure 6.0-7.0 MPa for 3-5 h at temperature 22-26 C followed by isolation of carbon dioxide extract. In the second stage carbon dioxide extract is treated with 70-94% ethyl alcohol solution at temperature 25-60 C for 5-10 min to yield alcoholic extract from which alcohol-soluble and alcohol-insoluble fractions are isolated. Prepared products of apiculture can be used in medicine, cosmetics, veterinary science and as independent medicinal agent. EFFECT: reduced fire hazard of method, stable products. 3 ex