METHOD FOR PREPARING INACTIVATED VACCINE AGAINST CATTLE LEUCOSIS Russian patent published in 2003 - IPC

FIELD: veterinary science. SUBSTANCE: the present method deals with cultivation of virus-containing peripheral blood leucocytes in leucosis-suffering cattle, inactivation and antigen obtaining. Cultivation of leucocytes is carried out for 24-36 h at inoculation concentration equal to 5-10×10⁶ cells/ml medium. Then leucocytes should be deposited due to centrifuging. Suspension should be prepared upon Eagle's medium at concentration of 10 mln cells/ml medium, leucocytes are fixed to inactivate the virus inside by adding 5 volumes of 2-3%-glutaric aldehyde solution. Incubation is carried out at room temperature for 30-40 min. Leucocytes should be thrice washed in buffered physiological solution at pH=7.2-7.4, resuspended in Eagle's medium at 100 mln leucocytes/ml to be used as immunizing agent. Vaccine obtained due to the suggested method provides accelerated terms of sanitation in leucosis-suffering farms by 2-3 times and decreased expenses necessary for sanitation. EFFECT: higher efficiency. 2 ex, 1 tbl